| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Francisco Álvarez | C | NYN | 23 | |
| Samuel Basallo | C | Bal | 21 | |
| Jonathon Long | 1B | ChN | 23 | |
| Kyle Manzardo | 1B | Cle | 25 | |
| Luke Keaschall | 2B | Min | 23 | September 25, 2025:10 Day IL |
| Brooks Lee | 2B (SS,3B) | Min | 24 | |
| Brice Matthews | 2B | Hou | 23 | |
| Christian Moore | 2B | LAA | 23 | |
| Ethan Holliday | SS | Col | 18 | |
| Ezequiel Tovar | SS | Col | 24 | |
| Carson Williams | SS | TB | 22 | |
| Jett Williams | SS | NYN | 21 | |
| Royce Lewis | 3B | Min | 26 | |
| Matt Shaw | 3B | ChN | 23 | |
| Jo Adell | OF | LAA | 26 | |
| Roman Anthony | OF | Bos | 21 | September 03, 2025:10 Day IL |
| Dylan Beavers | OF | Bal | N/A | |
| Lawrence Butler | OF | Oak | 25 | |
| Ryan Clifford | OF | NYN | N/A | |
| Dylan Crews | OF | Was | 23 | |
| Drew Gilbert | OF | SF | 25 | |
| Trent Grisham | OF | NYA | 28 | |
| Wenceel Perez | OF | Det | N/A | |
| Logan Allen | SP | Cle | 27 | |
| Chase Burns | SP (RP) | Cin | 22 | |
| Zac Gallen | SP | Ari | 30 | |
| Michael King | SP | SD | 30 | |
| Nolan McLean | SP | NYN | 24 | |
| Brandon Pfaadt | SP | Ari | 27 | |
| Roki Sasaki | SP | LAD | 23 | |
| Tarik Skubal | SP | Det | 28 | |
| Blake Snell | SP | LAD | 32 | |
| Payton Tolle | SP (RP) | Bos | 22 | |
| Ryne Nelson | RP (SP) | Ari | 27 |